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KMID : 0613820080180121723
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Journal of Life Science
2008 Volume.18 No. 12 p.1723 ~ p.1727
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Isolation and Identification of Chlorella sp. CMS-1 and the Chemical Composition of Its Hot Water Extract
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Cha Jae-Young
Kim Jung-Wook
Park Bo-Kyung
Jin Hyun-Jin
Kim Sung-Yong
Cho Young-Su
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Abstract
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CMS-1 strain was isolated from the outdoors cultivation pools in Culmansa Co., Ltd. This strain was found to be a rounded type of 3 ㎛. Phylogenetic analysis by the 18S rRNA sequencing using isolated strain is most similar to Chlorella sp. IFRPD 1018 gene at the level of nucleotide sequence identity at 99%. Accordingly, the isolated Chlorella strain was named as Chlorella sp. CMS-1 based on its morphological and phylogenetic properties. The concentrations of crude protein and fat were 59% and 0.01%, respectively. Major compositional amino acids (㎎%) were glutamic acid 6.21, alanine 5.76, aspartic acid 5.44%, glycine 4.29%, and threonine 3.09% and major free amino acids (㎎%) were γ-aminobutyric acid (GABA) 7.13%, L-alanine 1.44%, L-glutamic acid 0.90, L-leucine 0.26% and L-glycine 0.20%. The concentrations of major minerals were P 2.25%, K 2.25%, Na 1.09%, Mg 0.63%, and Ca 0.28%.
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KEYWORD
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Chlorella sp, CMS-1, amino acid, minerals, γ-aminobutyric acid(GABA)
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